Difference between revisions of "Lidstrom:Sorvall Centrifuge"

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(Centrifugation Math Basics)
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[[image:RCF_explanation.png|thumb|upright=2.0|center|RCF explanation]]
 
[[image:RCF_explanation.png|thumb|upright=2.0|center|RCF explanation]]
  
== Centrifugation Math Basics ==
+
== Centrifugation Math & Basics ==
 
* [http://www.sigmaaldrich.com/technical-documents/articles/biofiles/centrifugation-basics.html Sigma summary]:
 
* [http://www.sigmaaldrich.com/technical-documents/articles/biofiles/centrifugation-basics.html Sigma summary]:
 
** sedimentation rate is proportional to RFC, and proportional to the square of the particle diameter (cell diameter).  
 
** sedimentation rate is proportional to RFC, and proportional to the square of the particle diameter (cell diameter).  
 
** [[image:Lidstrom_Stokes_equation.png|thumb|upright=2.0|center|Stoke's equation]]
 
** [[image:Lidstrom_Stokes_equation.png|thumb|upright=2.0|center|Stoke's equation]]
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** [[image:Lidstrom centrifugation speeds for applications.png|thumb|upright=2.0|center|centrifugation speeds for applications]]

Revision as of 10:21, 24 October 2013

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Picture

Lidstrom Sorvoll centrifuge

Rotors

  • Sorvall rotors
    • Rotors we have:
      • SS-34. Holds 8*50 mL, spins at 20,000 RPM/36,900 RCF
      • GS-3.
      • ____. Holds eppendorf (1.5mL) tubes
  • For reference, the benchtop 50mL centrifuges (e.g. ST-15R with a TX-400 swinging bucket rotor) we have spin at 4696 RCF.
Lidstrom sorvoll speed instructions
SS-34 rotor speeds and k factors
GS-34 rotor speeds and k factors
RCF explanation

Centrifugation Math & Basics

  • Sigma summary:
    • sedimentation rate is proportional to RFC, and proportional to the square of the particle diameter (cell diameter).
    • Stoke's equation
    • centrifugation speeds for applications