# Difference between revisions of "Lidstrom:Sorvall Centrifuge"

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[[image:RCF_explanation.png|thumb|upright=2.0|center|RCF explanation]] | [[image:RCF_explanation.png|thumb|upright=2.0|center|RCF explanation]] | ||

− | == Centrifugation Math Basics == | + | == Centrifugation Math & Basics == |

* [http://www.sigmaaldrich.com/technical-documents/articles/biofiles/centrifugation-basics.html Sigma summary]: | * [http://www.sigmaaldrich.com/technical-documents/articles/biofiles/centrifugation-basics.html Sigma summary]: | ||

** sedimentation rate is proportional to RFC, and proportional to the square of the particle diameter (cell diameter). | ** sedimentation rate is proportional to RFC, and proportional to the square of the particle diameter (cell diameter). | ||

** [[image:Lidstrom_Stokes_equation.png|thumb|upright=2.0|center|Stoke's equation]] | ** [[image:Lidstrom_Stokes_equation.png|thumb|upright=2.0|center|Stoke's equation]] | ||

+ | ** [[image:Lidstrom centrifugation speeds for applications.png|thumb|upright=2.0|center|centrifugation speeds for applications]] |

## Revision as of 10:21, 24 October 2013

Back to Protocols

## Picture

## Rotors

- Sorvall rotors
- Rotors we have:
- SS-34. Holds 8*50 mL, spins at 20,000 RPM/36,900 RCF
- GS-3.
- ____. Holds eppendorf (1.5mL) tubes

- Rotors we have:
- For reference, the benchtop 50mL centrifuges (e.g. ST-15R with a TX-400 swinging bucket rotor) we have spin at 4696 RCF.

## Centrifugation Math & Basics

- Sigma summary:
- sedimentation rate is proportional to RFC, and proportional to the square of the particle diameter (cell diameter).