Difference between revisions of "Lidstrom:Sorvall Centrifuge"

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(Rotors)
(Rotors)
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== Rotors ==
 
== Rotors ==
* [http://www.geminibv.nl/labware/sorvall-gsa-rotor/sorvall-rotor-gsa-gs3-sa600-se12-sm24-ss34-manual.pdf Sorvall rotors]
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[http://www.geminibv.nl/labware/sorvall-gsa-rotor/sorvall-rotor-gsa-gs3-sa600-se12-sm24-ss34-manual.pdf Sorvall rotors]
** Rotors we have:
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*** SS-34.  Holds 8*50 mL, spins at 20,000 RPM/36,900 RCF
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Rotors we have:
****Tubes: [[image:Lidstrom_SS-34 tubes.png|thumb|upright=0.3|center|SS-34 tubes]] Can't be autoclaved.  Can't write on body; can only write on cap.  Sandy says syickers don't stick.  
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* SS-34.  Holds 8*50 mL, spins at 20,000 RPM/36,900 RCF
*** GS-3.
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**Tubes: [[image:Lidstrom_SS-34 tubes.png|thumb|upright=0.3|center|SS-34 tubes]]  
*** ____.  Holds eppendorf (1.5mL) tubes
+
***Can't be autoclaved.   
 +
**** Actually, Amanda might have autoclaved them before. 
 +
***Can't write on body; can only write on cap.  Sandy says stickers don't stick.  
 +
* [http://www.thermoscientific.fr/eThermo/CMA/PDFs/Various/File_582.pdf GS-3].
 +
** For bigger bottles.
 +
* ???.   
 +
** Holds eppendorf (1.5mL) tubes
 
* For reference, the benchtop 50mL centrifuges (e.g. [https://static.thermoscientific.com/images/D21714~.pdf ST-15R] with a [https://extranet.fisher.co.uk/webfiles/fr/Pjointes/Documentation/SOR007_EN%20SORVALL%20ST16&ST40%20CENTRIFUGE.pdf TX-400] swinging bucket rotor) we have spin at 4696 RCF.  
 
* For reference, the benchtop 50mL centrifuges (e.g. [https://static.thermoscientific.com/images/D21714~.pdf ST-15R] with a [https://extranet.fisher.co.uk/webfiles/fr/Pjointes/Documentation/SOR007_EN%20SORVALL%20ST16&ST40%20CENTRIFUGE.pdf TX-400] swinging bucket rotor) we have spin at 4696 RCF.  
  
There are stickers with maximum speeds.  These were set by Katya, circa 2011.  It is unknown what the motivation was for keeping the rotors below maximum speed.  
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*There are stickers with maximum speeds.  These were set by Katya, a Lidstrom Lab manager, circa 2011.  It is unknown what the motivation was for keeping the rotors below maximum speed.  
 
[[image:Lidstrom_sorvoll_speed_instructions.png|thumb|upright=2.0|center|Lidstrom sorvoll speed instructions]]
 
[[image:Lidstrom_sorvoll_speed_instructions.png|thumb|upright=2.0|center|Lidstrom sorvoll speed instructions]]
  

Revision as of 16:03, 11 June 2014

Back to Protocols


Picture

Lidstrom Sorvoll centrifuge

Rotors

Sorvall rotors

Rotors we have:

  • SS-34. Holds 8*50 mL, spins at 20,000 RPM/36,900 RCF
    • Tubes:
      SS-34 tubes
      • Can't be autoclaved.
        • Actually, Amanda might have autoclaved them before.
      • Can't write on body; can only write on cap. Sandy says stickers don't stick.
  • GS-3.
    • For bigger bottles.
  •  ???.
    • Holds eppendorf (1.5mL) tubes
  • For reference, the benchtop 50mL centrifuges (e.g. ST-15R with a TX-400 swinging bucket rotor) we have spin at 4696 RCF.
  • There are stickers with maximum speeds. These were set by Katya, a Lidstrom Lab manager, circa 2011. It is unknown what the motivation was for keeping the rotors below maximum speed.
Lidstrom sorvoll speed instructions
SS-34 rotor speeds and k factors
GS-34 rotor speeds and k factors
RCF explanation

Instructions

Example protocol for AM1:

Janet's SIP3-4 centrifugation:

  • Chill to 4oC with SS-34 rotor inside
  • Fill correct tubes
  • Spin for ___ min at ___ RCF at ___oC

Centrifugation Math & Basics

  • Sigma summary:
    • sedimentation rate is proportional to RFC, and proportional to the square of the particle diameter (cell diameter).
    • Stoke's equation
    • centrifugation speeds for applications