Difference between revisions of "Lidstrom:Protocols"

From OpenWetWare
Jump to: navigation, search
(DNA/RNA:)
(DNA)
Line 30: Line 30:
 
*[[Lidstrom:Overlap Extension PCR| Overlap Extension PCR (in progress)]]
 
*[[Lidstrom:Overlap Extension PCR| Overlap Extension PCR (in progress)]]
 
*[[Qiagen_Buffers| Qiagen Buffer Recipes]]
 
*[[Qiagen_Buffers| Qiagen Buffer Recipes]]
 +
 +
=====Fancy Manipulation=====
 +
*[[Lidstrom:Site-Directed Mutagenesis|Site-Directed Mutagenesis (in progress)]]
 +
*[[Lidstrom:λ-Red Mediated Gene Knockouts|λ-Red Mediated Gene Knockouts (in progress)]]
 +
*[[Dorman:P1_phage_%28lysogenic%29|Phage Lysate Prep]]
 +
*[[Lidstrom:P1 Phage Transduction|P1 Phage Transduction (in progress)]]
  
 
==== Restriction Cloning ====
 
==== Restriction Cloning ====
Line 37: Line 43:
 
==== Gibson Cloning ====
 
==== Gibson Cloning ====
 
*[[Lidstrom:Gibson Assembly| Gibson Assembly (In progress)]]
 
*[[Lidstrom:Gibson Assembly| Gibson Assembly (In progress)]]
 
=====Fancy Manipulation=====
 
*[[Lidstrom:Site-Directed Mutagenesis|Site-Directed Mutagenesis (in progress)]]
 
*[[Lidstrom:λ-Red Mediated Gene Knockouts|λ-Red Mediated Gene Knockouts (in progress)]]
 
*[[Dorman:P1_phage_%28lysogenic%29|Phage Lysate Prep]]
 
*[[Lidstrom:P1 Phage Transduction|P1 Phage Transduction (in progress)]]
 
  
 
====Obervation====
 
====Obervation====

Revision as of 06:07, 31 March 2013

Back home: Lidstrom

General Lab:

Cell Culture:

DNA/RNA:

DNA

Isolation

Manipulation

Fancy Manipulation

Restriction Cloning

Gibson Cloning

Obervation

Misc.

Protein

E. Coli

Enzyme Assays

General

in vitro pathway assays (CO2 project)

Metabolomics

CO2 project