Difference between revisions of "Lidstrom:Protocols"

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(Assays)
(Cell Culture:)
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*[[Lidstrom:Preparing Freezer Cell Stocks| Preparing Freezer Cell Stocks]]
 
*[[Lidstrom:Preparing Freezer Cell Stocks| Preparing Freezer Cell Stocks]]
 
*[[Lidstrom:Pouring Media Plates|Pouring Media Plates]]
 
*[[Lidstrom:Pouring Media Plates|Pouring Media Plates]]
 +
*[[Lidstrom:Filtration|Filtration]]
  
 
==Cell Culture:==
 
==Cell Culture:==
 
*[[Lidstrom:Tube Spec|Tube Spec]]
 
*[[Lidstrom:Tube Spec|Tube Spec]]
 
*[[Lidstrom: Bioscreen|Bioscreen Setup]]
 
*[[Lidstrom: Bioscreen|Bioscreen Setup]]
*[[Lidstrom:NanoDrop|Nano Drop for Cell Cultures]]
+
 
 
=== Methylotrophs ===
 
=== Methylotrophs ===
 
*[[Lidstrom:MM1/MM2/MM3/HY|MM1/MM2/MM3/HY]]
 
*[[Lidstrom:MM1/MM2/MM3/HY|MM1/MM2/MM3/HY]]
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*[[Lidstrom:TB|Terrific Broth recipe]]
 
*[[Lidstrom:TB|Terrific Broth recipe]]
 
*[[Lidstrom: EZ amino acid stock recipe|EZ amino acid stock recipe]]
 
*[[Lidstrom: EZ amino acid stock recipe|EZ amino acid stock recipe]]
 +
 +
=== OD measurements ===
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* [[Lidstrom:Beckmann DU 640B spectrophotometer|Beckmann DU 640B spectrophotometer]]
 +
*[[Lidstrom:NanoDrop|Nano Drop for Cell Cultures]]
  
 
==DNA/RNA:==
 
==DNA/RNA:==
  
 
===DNA===
 
===DNA===
== PCR ==  
+
==== PCR ====  
 
*[[Lidstrom:Colony PCR| Colony PCR]]
 
*[[Lidstrom:Colony PCR| Colony PCR]]
 
*[[Lidstrom:PCR| PCR (in progress)]]
 
*[[Lidstrom:PCR| PCR (in progress)]]
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*[[Lidstrom:Oligo Orders | Oligo Orders]]
 
*[[Lidstrom:Oligo Orders | Oligo Orders]]
 
*[[Lidstrom:Diluting Primers | Diluting Primers]]
 
*[[Lidstrom:Diluting Primers | Diluting Primers]]
 +
*[https://www.neb.com/~/media/NebUs/Files/Brochures/Cloning_Guide_1113.pdf NEB cloning guide]
  
 
==Protein==
 
==Protein==
 
*[[Lidstrom: SDS-PAGE | SDS-PAGE Protein Gels]]
 
*[[Lidstrom: SDS-PAGE | SDS-PAGE Protein Gels]]
 +
 +
=== Protein Concentration Determination ===
 +
*[[Lidstrom:Choosing a protein concentration quantification method|Choosing a protein concentration quantification method]]
 
*[[Lidstrom:BCA assay|BCA assay]] to determine protein concentration
 
*[[Lidstrom:BCA assay|BCA assay]] to determine protein concentration
 +
*[[Lidstrom:NanoDrop#NanoDrop_for_Proteins|NanoDrop for proteins]]
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 +
== Chemicals ==
 +
*[[Lidstrom:Chemical Stability|Chemical Stability]]
 +
* Also, see [[Lidstrom:Solution Stock Info|Solution Stock Info]]
  
 
==Buffers==
 
==Buffers==
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==Assays==
 
==Assays==
*[[Lidstrom:Sorvall Centrifuge|Sorvall centrifuge]]  goes faster than the regular centrifuges that allow 50mL tubes
+
 
 
===General===
 
===General===
*[[Lidstrom: Molecular Devices Plate Reader| Molecular Devices (beige) Plate Reader Setup]]
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*[[Lidstrom:Enzyme Assay Basics|Enzyme Assay Basics]]
*[[Lidstrom: Tecan Plate Reader|Tecan (dark grey) Plate Reader]]
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*[[Lidstrom:Solution Stock Info|Solution Stock Info]]
*[[Lidstrom: Flow Cytometer| Flow Cytometer]]
+
 
 +
=== Equipment ===
 +
*Plate readers:
 +
**[[Lidstrom: Molecular Devices Plate Reader| Molecular Devices (beige) Plate Reader Setup]]
 +
**[[Lidstrom: Tecan Plate Reader|Tecan (dark grey) Plate Reader]]
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* Cuvette spectrophotometer:
 +
** [[Lidstrom:UV-2401PC UV-Vis recording spectrophotometer|UV-2401PC UV-Vis recording spectrophotometer]]
 +
* Centrifuge:
 +
**[[Lidstrom:Sorvall Centrifuge|Sorvall centrifuge]]  goes faster than the regular centrifuges that allow 50mL tubes
 +
* Flow Cytometry:
 +
**[[Lidstrom: Flow Cytometer| Flow Cytometer]]
  
 
===in vitro pathway assays (CO<sub>2</sub> project)===
 
===in vitro pathway assays (CO<sub>2</sub> project)===
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*[[Lidstrom:in vitro Pathway Assay: Crude Extract Prep| Crude Extract Prep (in progress)]]
 
*[[Lidstrom:in vitro Pathway Assay: Crude Extract Prep| Crude Extract Prep (in progress)]]
 
*[[Lidstrom:in vitro Pathway Assay: Sample Analysis via UPLC-MS/MS | Sample Analysis via UPLC-MS/MS (in progress)]]
 
*[[Lidstrom:in vitro Pathway Assay: Sample Analysis via UPLC-MS/MS | Sample Analysis via UPLC-MS/MS (in progress)]]
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 +
==== Misc ====
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*[[Lidstrom:Assaying enzyme libraries in 96-well plates|Assaying enzyme libraries in 96-well plates]]
  
 
== Metabolomics ==
 
== Metabolomics ==

Revision as of 16:23, 25 March 2014

Back home: Lidstrom

General Lab:

Cell Culture:

Methylotrophs

E. coli

OD measurements

DNA/RNA:

DNA

PCR

Isolation

Manipulation

Fancy Manipulation

Restriction Cloning

Gibson Cloning

Obervation

Misc.

Protein

Protein Concentration Determination

Chemicals

Buffers

E. coli

E. coli transformation

Lysing & Cells & Clarifying Extract

Assays

General

Equipment

in vitro pathway assays (CO2 project)

Misc

Metabolomics

CO2 project