Lidstrom:Protocols: Difference between revisions
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==Protein== | ==Protein== | ||
*[[Lidstrom: SDS-PAGE | SDS-PAGE Protein Gels]] | *[[Lidstrom: SDS-PAGE | SDS-PAGE Protein Gels]] | ||
=== Protein Concentration Determination === | |||
*[[Lidstrom:Choosing a protein concentration quantification method|Choosing a protein concentration quantification method]] | |||
*[[Lidstrom:BCA assay|BCA assay]] to determine protein concentration | *[[Lidstrom:BCA assay|BCA assay]] to determine protein concentration | ||
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*[[Lidstrom:Buffers|Buffers]] | *[[Lidstrom:Buffers|Buffers]] | ||
==E. | ==E. coli== | ||
*[[Lidstrom:E. Coli Vector Compatibility| E. | *[[Lidstrom:E. Coli Vector Compatibility| E. coli Vector Compatibility (In Progress)]] | ||
*[[Lidstrom:Competent Cell Preparation|Competent Cell Preparation (in progress)]] | *[[Lidstrom:Competent Cell Preparation|Competent Cell Preparation (in progress)]] | ||
=== E. coli transformation === | |||
*[[Lidstrom:Chemical Transformation|Chemical Transformation]] | *[[Lidstrom:Chemical Transformation|Chemical Transformation]] | ||
*[[Lidstrom:Electroporation|Electroporation]] | *[[Lidstrom:Electroporation|Electroporation]] | ||
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===General=== | ===General=== | ||
*[[Lidstrom:Enzyme Assay Basics|Enzyme Assay Basics]] | |||
=== Equipment === | |||
*[[Lidstrom: Molecular Devices Plate Reader| Molecular Devices (beige) Plate Reader Setup]] | *[[Lidstrom: Molecular Devices Plate Reader| Molecular Devices (beige) Plate Reader Setup]] | ||
*[[Lidstrom: Tecan Plate Reader|Tecan (dark grey) Plate Reader]] | *[[Lidstrom: Tecan Plate Reader|Tecan (dark grey) Plate Reader]] | ||
*[[Lidstrom:Sorvall Centrifuge|Sorvall centrifuge]] goes faster than the regular centrifuges that allow 50mL tubes | |||
*[[Lidstrom: Flow Cytometer| Flow Cytometer]] | *[[Lidstrom: Flow Cytometer| Flow Cytometer]] | ||
Revision as of 15:40, 31 October 2013
Back home: Lidstrom
General Lab:
- Health & Safety
- Finding Chemicals
- Dishwasher
- Autoclave
- Sterile technique
- E. Coli basics
- Preparing Freezer Cell Stocks
- Pouring Media Plates
Cell Culture:
Methylotrophs
E. coli
DNA/RNA:
DNA
PCR
Isolation
- QIAquick Gel Extraction Kit Protocol
- Genomic DNA minikit extraction
- Genomic DNA phenol/chloroform extraction
Manipulation
Fancy Manipulation
- Site-Directed Mutagenesis (in progress)
- λ-Red Mediated Gene Knockouts (in progress)
- Phage Lysate Prep
- P1 Phage Transduction (in progress)
Restriction Cloning
Gibson Cloning
Obervation
Misc.
Protein
Protein Concentration Determination
- Choosing a protein concentration quantification method
- BCA assay to determine protein concentration
Buffers
E. coli
E. coli transformation
Lysing & Cells & Clarifying Extract
Assays
General
Equipment
- Sorvall centrifuge goes faster than the regular centrifuges that allow 50mL tubes
in vitro pathway assays (CO2 project)
- Cell Prep (in progress)
- Crude Extract Prep (in progress)
- Sample Analysis via UPLC-MS/MS (in progress)
