Difference between revisions of "Lidstrom:Protocols"

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(Lysing Cells)
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===DNA===
 
===DNA===
 +
== PCR ==
 +
*[[Lidstrom:Colony PCR| Colony PCR]]
 +
*[[Lidstrom:PCR| PCR (in progress)]]
 +
*[[Lidstrom:Thermocyclers|Thermocyclers]]
 +
 
====Isolation ====
 
====Isolation ====
 
*[[Lidstrom:QIAquick Gel Extraction Kit Protocol|QIAquick Gel Extraction Kit Protocol]]
 
*[[Lidstrom:QIAquick Gel Extraction Kit Protocol|QIAquick Gel Extraction Kit Protocol]]
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====Misc.====
 
====Misc.====
*[[Lidstrom:PCR| PCR (in progress)]]
 
 
*[[Lidstrom:Loading Dye Recipe|Loading Dye Recipe]]
 
*[[Lidstrom:Loading Dye Recipe|Loading Dye Recipe]]
 
*[[Lidstrom:Purifying DNA|Purifying DNA (in progress)]]
 
*[[Lidstrom:Purifying DNA|Purifying DNA (in progress)]]
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==Protein==
 
==Protein==
 
*[[Lidstrom: SDS-PAGE | SDS-PAGE Protein Gels]]
 
*[[Lidstrom: SDS-PAGE | SDS-PAGE Protein Gels]]
 +
*[[Lidstrom:BCA assay|BCA assay]] to determine protein concentration
  
 
==Buffers==
 
==Buffers==
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*[[Lidstrom:Competent Cells|Competent Cell Lines]]
 
*[[Lidstrom:Competent Cells|Competent Cell Lines]]
  
== Lysing Cells ==
+
== Lysing & Cells & Clarifying Extract ==
 
*[[Lidstrom:French Press|French Press]]
 
*[[Lidstrom:French Press|French Press]]
*[[Lidstrom:Sonicator|Sonicator]]
+
*[[Lidstrom:Sonicator|Sonication]]
 +
*[[Lidstrom:Ultracentrifuge|Ultracentrifuge]]
  
==Enzyme Assays==
+
==Assays==
  
 
===General===
 
===General===
 
*[[Lidstrom: Plate Reader| Plate Reader Setup]]
 
*[[Lidstrom: Plate Reader| Plate Reader Setup]]
 +
*[[Lidstrom: Flow Cytometer| Flow Cytometer]]
  
 
===in vitro pathway assays (CO<sub>2</sub> project)===
 
===in vitro pathway assays (CO<sub>2</sub> project)===

Revision as of 11:57, 28 June 2013

Back home: Lidstrom

General Lab:

Cell Culture:

Methylotrophs

E. coli

DNA/RNA:

DNA

PCR

Isolation

Manipulation

Fancy Manipulation

Restriction Cloning

Gibson Cloning

Obervation

Misc.

Protein

Buffers

E. Coli

Lysing & Cells & Clarifying Extract

Assays

General

in vitro pathway assays (CO2 project)

Metabolomics

CO2 project