Difference between revisions of "Lidstrom:Protocols"

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(General Lab:)
(Cell Culture:)
(7 intermediate revisions by 3 users not shown)
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*[[Lidstrom:Preparing Freezer Cell Stocks| Preparing Freezer Cell Stocks]]
 
*[[Lidstrom:Preparing Freezer Cell Stocks| Preparing Freezer Cell Stocks]]
 
*[[Lidstrom:Pouring Media Plates|Pouring Media Plates]]
 
*[[Lidstrom:Pouring Media Plates|Pouring Media Plates]]
*[[Lidstrom:Ordering | Ordering]]
 
  
 
==Cell Culture:==
 
==Cell Culture:==
 
*[[Lidstrom:Tube Spec|Tube Spec]]
 
*[[Lidstrom:Tube Spec|Tube Spec]]
 +
*[[Lidstrom: Bioscreen|Bioscreen Setup]]
 +
=== Methylotrophs ===
 +
*[[Lidstrom:MM1/MM2/MM3/HY|MM1/MM2/MM3/HY]]
 +
===E. coli === 
 
*[[Lidstrom:M9|M9 recipe]]
 
*[[Lidstrom:M9|M9 recipe]]
 
*[[Lidstrom:TB|Terrific Broth recipe]]
 
*[[Lidstrom:TB|Terrific Broth recipe]]
*[[Lidstrom: Bioscreen|Bioscreen Setup]]
+
*[[Lidstrom: EZ amino acid stock recipe|EZ amino acid stock recipe]]
  
 
==DNA/RNA:==
 
==DNA/RNA:==
  
 
===DNA===
 
===DNA===
 +
====Isolation ====
 +
*[[Lidstrom:QIAquick Gel Extraction Kit Protocol|QIAquick Gel Extraction Kit Protocol]]
 +
*[[Lidstrom:Genomic DNA extraction|Genomic DNA minikit extraction]]
 +
*[[Lidstrom:Genomic DNA extraction|Genomic DNA phenol/chloroform extraction]]
  
 
====Manipulation====
 
====Manipulation====
 
*[[Lidstrom:Building with DNA|Intro: Building with DNA (in progress)]]
 
*[[Lidstrom:Building with DNA|Intro: Building with DNA (in progress)]]
*[[Lidstrom:Digestion with Restriction Enzymes|Digestion with Restriction Enzymes]]
 
*[[Lidstrom:Gibson Assembly| Gibson Assembly (In progress)]]
 
*[[Lidstrom:Ligation |Ligation (in progress)]]
 
 
*[[Lidstrom:Overlap Extension PCR| Overlap Extension PCR (in progress)]]
 
*[[Lidstrom:Overlap Extension PCR| Overlap Extension PCR (in progress)]]
 
*[[Qiagen_Buffers| Qiagen Buffer Recipes]]
 
*[[Qiagen_Buffers| Qiagen Buffer Recipes]]
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*[[Dorman:P1_phage_%28lysogenic%29|Phage Lysate Prep]]
 
*[[Dorman:P1_phage_%28lysogenic%29|Phage Lysate Prep]]
 
*[[Lidstrom:P1 Phage Transduction|P1 Phage Transduction (in progress)]]
 
*[[Lidstrom:P1 Phage Transduction|P1 Phage Transduction (in progress)]]
 +
 +
==== Restriction Cloning ====
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*[[Lidstrom:Digestion with Restriction Enzymes|Digestion with Restriction Enzymes]]
 +
*[[Lidstrom:Ligation |Ligation (in progress)]]
 +
 +
==== Gibson Cloning ====
 +
*[[Lidstrom:Gibson Assembly| Gibson Assembly (In progress)]]
  
 
====Obervation====
 
====Obervation====

Revision as of 14:24, 2 May 2013

Back home: Lidstrom

General Lab:

Cell Culture:

Methylotrophs

E. coli

DNA/RNA:

DNA

Isolation

Manipulation

Fancy Manipulation

Restriction Cloning

Gibson Cloning

Obervation

Misc.

Protein

E. Coli

Enzyme Assays

General

in vitro pathway assays (CO2 project)

Metabolomics

CO2 project