Difference between revisions of "Lidstrom:Protocols"

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(DNA/RNA:)
(DNA)
(16 intermediate revisions by 3 users not shown)
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*[[Lidstrom:TB|Terrific Broth recipe]]
 
*[[Lidstrom:TB|Terrific Broth recipe]]
 
*[[Lidstrom: Bioscreen|Bioscreen Setup]]
 
*[[Lidstrom: Bioscreen|Bioscreen Setup]]
 +
*[[Lidstrom: EZ amino acid stock recipe|EZ amino acid stock recipe]]
  
 
==DNA/RNA:==
 
==DNA/RNA:==
  
 
===DNA===
 
===DNA===
 +
====Isolation ====
 +
*[[Lidstrom:QIAquick Gel Extraction Kit Protocol|QIAquick Gel Extraction Kit Protocol]]
 +
*[[Lidstrom:Genomic DNA extraction|Genomic DNA minikit extraction]]
 +
*[[Lidstrom:Genomic DNA extraction|Genomic DNA phenol/chloroform extraction]]
  
 
====Manipulation====
 
====Manipulation====
 
*[[Lidstrom:Building with DNA|Intro: Building with DNA (in progress)]]
 
*[[Lidstrom:Building with DNA|Intro: Building with DNA (in progress)]]
*[[Lidstrom:Digestion with Restriction Enzymes|Digestion with Restriction Enzymes]]
 
*[[Lidstrom:Gibson Assembly| Gibson Assembly (In progress)]]
 
*[[Lidstrom:Ligation |Ligation (in progress)]]
 
 
*[[Lidstrom:Overlap Extension PCR| Overlap Extension PCR (in progress)]]
 
*[[Lidstrom:Overlap Extension PCR| Overlap Extension PCR (in progress)]]
 +
*[[Qiagen_Buffers| Qiagen Buffer Recipes]]
  
 
=====Fancy Manipulation=====
 
=====Fancy Manipulation=====
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*[[Dorman:P1_phage_%28lysogenic%29|Phage Lysate Prep]]
 
*[[Dorman:P1_phage_%28lysogenic%29|Phage Lysate Prep]]
 
*[[Lidstrom:P1 Phage Transduction|P1 Phage Transduction (in progress)]]
 
*[[Lidstrom:P1 Phage Transduction|P1 Phage Transduction (in progress)]]
 +
 +
==== Restriction Cloning ====
 +
*[[Lidstrom:Digestion with Restriction Enzymes|Digestion with Restriction Enzymes]]
 +
*[[Lidstrom:Ligation |Ligation (in progress)]]
 +
 +
==== Gibson Cloning ====
 +
*[[Lidstrom:Gibson Assembly| Gibson Assembly (In progress)]]
  
 
====Obervation====
 
====Obervation====
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*[[Lidstrom:Purifying DNA|Purifying DNA (in progress)]]
 
*[[Lidstrom:Purifying DNA|Purifying DNA (in progress)]]
 
*[[Lidstrom:Miniprep |Miniprep (in progress)]]
 
*[[Lidstrom:Miniprep |Miniprep (in progress)]]
*[[Lidstrom:Oligo Orders | Oligo Orders (in progress)]]
+
*[[Lidstrom:Oligo Orders | Oligo Orders]]
 
*[[Lidstrom:Diluting Primers | Diluting Primers]]
 
*[[Lidstrom:Diluting Primers | Diluting Primers]]
  
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==E. Coli==
 
==E. Coli==
 +
*[[Lidstrom:E. Coli Vector Compatibility| E. Coli Vector Compatibility  (In Progress)]]
 
*[[Lidstrom:Competent Cell Preparation|Competent Cell Preparation (in progress)]]
 
*[[Lidstrom:Competent Cell Preparation|Competent Cell Preparation (in progress)]]
 
*[[Lidstrom:Chemical Transformation|Chemical Transformation]]
 
*[[Lidstrom:Chemical Transformation|Chemical Transformation]]
 
*[[Lidstrom:Electroporation|Electroporation]]
 
*[[Lidstrom:Electroporation|Electroporation]]
 +
*[[Lidstrom:Competent Cells|Competent Cell Lines]]
  
 
==Enzyme Assays==
 
==Enzyme Assays==
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== Metabolomics ==
 
== Metabolomics ==
 +
=== CO2 project ===
 
*[[Lidstrom:Measuring 13C Incorporation Into Protein - CO2 Project|Measuring 13C Incorporation Into Protein - CO2 Project]]
 
*[[Lidstrom:Measuring 13C Incorporation Into Protein - CO2 Project|Measuring 13C Incorporation Into Protein - CO2 Project]]
 +
** [[Lidstrom:13C Incorporation Into Protein - Data Analysis]]

Revision as of 06:07, 31 March 2013

Back home: Lidstrom

General Lab:

Cell Culture:

DNA/RNA:

DNA

Isolation

Manipulation

Fancy Manipulation

Restriction Cloning

Gibson Cloning

Obervation

Misc.

Protein

E. Coli

Enzyme Assays

General

in vitro pathway assays (CO2 project)

Metabolomics

CO2 project