Difference between revisions of "Lidstrom:Protocols"

From OpenWetWare
Jump to: navigation, search
m (Protein)
(Fancy Manipulation)
Line 47: Line 47:
 
=====Fancy Manipulation=====
 
=====Fancy Manipulation=====
 
*[[Lidstrom:Site-Directed Mutagenesis|Site-Directed Mutagenesis (in progress)]]
 
*[[Lidstrom:Site-Directed Mutagenesis|Site-Directed Mutagenesis (in progress)]]
 +
** [[Lidstrom:QuikChange Site-Directed Mutagenesis|QuikChange Site-Directed Mutagenesis]]
 
*[[Lidstrom:λ-Red Mediated Gene Knockouts|λ-Red Mediated Gene Knockouts (in progress)]]
 
*[[Lidstrom:λ-Red Mediated Gene Knockouts|λ-Red Mediated Gene Knockouts (in progress)]]
 
*[[Dorman:P1_phage_%28lysogenic%29|Phage Lysate Prep]]
 
*[[Dorman:P1_phage_%28lysogenic%29|Phage Lysate Prep]]

Revision as of 05:51, 12 June 2014

Back home: Lidstrom

General Lab:

Cell Culture:

Methylotrophs

E. coli

OD measurements

DNA/RNA:

DNA

PCR

Isolation

Manipulation

Fancy Manipulation

Restriction Cloning

Gibson Cloning

Obervation

Misc.

Protein

Purification

Analytics

Protein Concentration Determination

Chemicals

Buffers

E. coli

E. coli transformation

Lysing & Cells & Clarifying Extract

Assays

General

Equipment

in vitro pathway assays (CO2 project)

Misc

Metabolomics

CO2 project