Lidstrom:Protocols: Difference between revisions
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*[[Lidstrom:Overlap Extension PCR| Overlap Extension PCR (in progress)]] | *[[Lidstrom:Overlap Extension PCR| Overlap Extension PCR (in progress)]] | ||
*[[Qiagen_Buffers| Qiagen Buffer Recipes]] | *[[Qiagen_Buffers| Qiagen Buffer Recipes]] | ||
=====Fancy Manipulation===== | |||
*[[Lidstrom:Site-Directed Mutagenesis|Site-Directed Mutagenesis (in progress)]] | |||
*[[Lidstrom:λ-Red Mediated Gene Knockouts|λ-Red Mediated Gene Knockouts (in progress)]] | |||
*[[Dorman:P1_phage_%28lysogenic%29|Phage Lysate Prep]] | |||
*[[Lidstrom:P1 Phage Transduction|P1 Phage Transduction (in progress)]] | |||
==== Restriction Cloning ==== | ==== Restriction Cloning ==== | ||
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==== Gibson Cloning ==== | ==== Gibson Cloning ==== | ||
*[[Lidstrom:Gibson Assembly| Gibson Assembly (In progress)]] | *[[Lidstrom:Gibson Assembly| Gibson Assembly (In progress)]] | ||
====Obervation==== | ====Obervation==== |
Revision as of 07:07, 31 March 2013
Back home: Lidstrom
General Lab:
- Health & Safety
- Finding Chemicals
- Dishwasher
- Autoclave
- Sterile technique
- E. Coli basics
- Preparing Freezer Cell Stocks
- Pouring Media Plates
Cell Culture:
DNA/RNA:
DNA
Isolation
- QIAquick Gel Extraction Kit Protocol
- Genomic DNA minikit extraction
- Genomic DNA phenol/chloroform extraction
Manipulation
Fancy Manipulation
- Site-Directed Mutagenesis (in progress)
- λ-Red Mediated Gene Knockouts (in progress)
- Phage Lysate Prep
- P1 Phage Transduction (in progress)
Restriction Cloning
Gibson Cloning
Obervation
Misc.
- PCR (in progress)
- Loading Dye Recipe
- Purifying DNA (in progress)
- Miniprep (in progress)
- Oligo Orders
- Diluting Primers
Protein
E. Coli
- E. Coli Vector Compatibility (In Progress)
- Competent Cell Preparation (in progress)
- Chemical Transformation
- Electroporation
- Competent Cell Lines
Enzyme Assays
General
in vitro pathway assays (CO2 project)
- Cell Prep (in progress)
- Crude Extract Prep (in progress)
- Sample Analysis via UPLC-MS/MS (in progress)