Difference between revisions of "Lidstrom:Preparing Freezer Cell Stocks"
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Revision as of 18:28, 10 February 2012
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- Grow ~ 5 mL of E. Coli overnight with appropriate antibiotics.
- Centrifuge for 5-10 minutes & pour off supernatant.
- Resuspend in 900 uL of HY (hypho) and 100 uL of filter-sterilized DMSO.
- Store at -80oC.
- Test stock after frozen a while.