Difference between revisions of "Lidstrom:Preparing Freezer Cell Stocks"

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==Glycerol==
 
==Glycerol==
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Revision as of 18:28, 10 February 2012

Back to protocols

DMSO

  • Grow ~ 5 mL of E. Coli overnight with appropriate antibiotics.
  • Centrifuge for 5-10 minutes & pour off supernatant.
  • Resuspend in 900 uL of HY (hypho) and 100 uL of filter-sterilized DMSO.
  • Store at -80oC.
  • Test stock after frozen a while.

Glycerol