Lidstrom:Gibson Assembly

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Revision as of 07:39, 24 February 2012 by Janet B. Matsen (talk | contribs)
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Design: - overlap by 40 bp

Misc: - rxn product is salty: can be a problem for electrocompetent cells. Rob Egbert dilutes the competent cells when transforming with electroporation. Not so much a problem when using chemically competent cells. - Electrocompetence is ~ 1000x more effective so is tempting. -