Difference between revisions of "Lidstrom:Gibson Assembly"

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(New page: Design: - overlap by 20 bp Misc: - rxn product is salty: can be a problem for electrocompetent cells. Rob Egbert dilutes the competent cells when transforming with electroporation. Not ...)
 
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Design:
 
Design:
- overlap by 20 bp
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- overlap by 40 bp
  
 
Misc:
 
Misc:

Revision as of 06:39, 24 February 2012

Design: - overlap by 40 bp

Misc: - rxn product is salty: can be a problem for electrocompetent cells. Rob Egbert dilutes the competent cells when transforming with electroporation. Not so much a problem when using chemically competent cells. - Electrocompetence is ~ 1000x more effective so is tempting. -