Lidstrom:Digestion with Restriction Enzymes

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Revision as of 14:02, 2 October 2012 by Janet B. Matsen (talk | contribs) (Temperature/Time)
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Recipe

  • If using BioBrick or BglBrick enzymes, chose your buffer based on Janet's notes.

Temperature/Time

  • 1 - 2 hours at 37oC or room temperature overnight
  • Inactivate by ..