Difference between revisions of "Lidstrom:Digestion with Restriction Enzymes"

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== Recipe ==
 
== Recipe ==
 
*Try it quick and imprecise like [http://2011.igem.org/Team:Washington/Protocols/Digestion iGEM 2011 UW] recommends:
 
*Try it quick and imprecise like [http://2011.igem.org/Team:Washington/Protocols/Digestion iGEM 2011 UW] recommends:
**10 uL DNA
+
**10 uL DNA (totaling 500+ ng is good)
 
**5uL buffer (check http://www.neb.com/nebecomm/EnzymeFinderSearchByName.asp for a list of enzymes and associated buffers)
 
**5uL buffer (check http://www.neb.com/nebecomm/EnzymeFinderSearchByName.asp for a list of enzymes and associated buffers)
 
**0.5 uL BSA (if required. See above)
 
**0.5 uL BSA (if required. See above)

Revision as of 14:54, 2 October 2012

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Recipe

  • If using BioBrick or BglBrick enzymes, chose your buffer based on Janet's notes.

Temperature/Time