Lidstrom:Choosing a protein concentration quantification method: Difference between revisions
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Back to [[Lidstrom:Protocols|Protocols]] | Back to [[Lidstrom:Protocols|Protocols]] | ||
=== Major methods available === | |||
* Spectrophotometer: use a formula based on A260 and A230 (260 and 230 nm absorbances) | |||
** fastest/easiest but may have most bias (??) | |||
** Ceci/Amanda/Frances chose BCA instead. | |||
* Pierce brand BCA assay | |||
=== Compatibility with your lysis solution === | === Compatibility with your lysis solution === | ||
[[image:Protein Assay Compatibility Table.png|thumb|upright=3.0|center|protein assay compatibility table from [http://compbio.korea.ac.kr/wiki/images/0/0e/PierceBCA.pdf Pierce]]] | [[image:Protein Assay Compatibility Table.png|thumb|upright=3.0|center|protein assay compatibility table from [http://compbio.korea.ac.kr/wiki/images/0/0e/PierceBCA.pdf Pierce]]] | ||
Revision as of 06:38, 30 October 2013
Back to Protocols
Major methods available
- Spectrophotometer: use a formula based on A260 and A230 (260 and 230 nm absorbances)
- fastest/easiest but may have most bias (??)
- Ceci/Amanda/Frances chose BCA instead.
- Pierce brand BCA assay
Compatibility with your lysis solution
