Lidstrom:Choosing a protein concentration quantification method: Difference between revisions
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* [http://www.piercenet.com/method/overview-protein-assays Thermo Scientific: Overview of Protein Assays Methods] | * [http://www.piercenet.com/method/overview-protein-assays Thermo Scientific: Overview of Protein Assays Methods] | ||
* [http://www.piercenet.com/guide/protein-assay-selection-guide Thermo Scientific: Protein Assay Selection Guide] | * [http://www.piercenet.com/guide/protein-assay-selection-guide Thermo Scientific: Protein Assay Selection Guide] | ||
* [http://www.piercenet.com/page/protein-assay-handbook-1602063 Thermo Scientific: Protein Assay Technical Handbook] | |||
Revision as of 07:24, 30 October 2013
Back to Protocols
Major methods available
- Spectrophotometer: use a formula based on A260 and A230 (260 and 230 nm absorbances)
- fastest/easiest but may have most bias (??)
- Ceci/Amanda/Frances chose BCA instead.
- Pierce brand BCA assay
Compatibility with your lysis solution
