Difference between revisions of "Lidstrom:Choosing a protein concentration quantification method"

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Back to [[Lidstrom:Protocols|Protocols]]
 
Back to [[Lidstrom:Protocols|Protocols]]
  
=== Major methods available ===
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== Major methods available ==
 
* Spectrophotometer: use a formula based on A260 and A230 (260 and 230 nm absorbances)
 
* Spectrophotometer: use a formula based on A260 and A230 (260 and 230 nm absorbances)
 
** fastest/easiest but may have most bias (??)  
 
** fastest/easiest but may have most bias (??)  
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* Pierce brand BCA assay
 
* Pierce brand BCA assay
  
=== Compatibility with your lysis solution ===
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== Compatibility with your lysis solution ==
 
[[image:Protein Assay Compatibility Table.png|thumb|upright=3.0|center|protein assay compatibility table from [http://compbio.korea.ac.kr/wiki/images/0/0e/PierceBCA.pdf Pierce]]]
 
[[image:Protein Assay Compatibility Table.png|thumb|upright=3.0|center|protein assay compatibility table from [http://compbio.korea.ac.kr/wiki/images/0/0e/PierceBCA.pdf Pierce]]]
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== Resources ==
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* [http://www.piercenet.com/method/overview-protein-assays Thermo Scientific: Overview of Protein Assays Methods]
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* [http://www.piercenet.com/guide/protein-assay-selection-guide Thermo Scientific: Protein Assay Selection Guide]

Revision as of 06:24, 30 October 2013

Back to Protocols

Major methods available

  • Spectrophotometer: use a formula based on A260 and A230 (260 and 230 nm absorbances)
    • fastest/easiest but may have most bias (??)
    • Ceci/Amanda/Frances chose BCA instead.
  • Pierce brand BCA assay

Compatibility with your lysis solution

protein assay compatibility table from Pierce

Resources