Lidstrom:Autoclave: Difference between revisions
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Back to [[Lidstrom:Protocols|Protocols]] | Back to [[Lidstrom:Protocols|Protocols]] | ||
== Large Autoclave (Room 435: by media prep room) == | |||
*Ask Lab Manager for instruction before using for first time! | |||
*Do not open door during a run. Escaping Steam presents a burn risk! | |||
*If there is an emergency, turn autoclave off and leave it to vent itself! | |||
*Always use log book | |||
#Turn autoclave on | |||
#Select cycle | |||
##Cycle 1: Media less than 1L | |||
##Cycle 2: Media 1L or more | |||
##Cycle 3: Glassware | |||
##Cycle 4: Biological waste | |||
#Put items into autoclave in Nalgene autoclavable trays | |||
#Close door | |||
#Wait for ready light to come on | |||
#Press start button | |||
#Do not open door until cycle is fully complete | |||
#Open door a crack to and let stem vent | |||
#Turn off autoclave when finished | |||
== Small Autoclave (Room 409 by RNA room) == | |||
*Ask Lab Manager for instruction before using for first time! | |||
*Do not open door during a run. Escaping Steam presents a burn risk! | |||
*If there is an emergency, turn autoclave off and leave it to vent itself! | |||
#Make sure that the internal water level inside the autoclave chamber is even with top of metal platform. | |||
##If it is not fill with dH2O until it is. This will ensure your samples get autoclaved, and not burnt. | |||
This will ensure your samples get autoclaved, and not burnt. | #Make sure external reservoir is filled so that the water level is between the high and low mark | ||
##If it is not fill with dH2O or empty so that it is | |||
#Place samples to be autoclaved into the chamber using metal cages to stack multiple samples | |||
#Shut door and turn wheel until door is firmly locked | |||
#Place magnetic key into receptacle | |||
#Turn autoclave on | |||
#Adjust time and temperature | |||
#Press the start button | |||
#Wait till run is completely finished before opening door | |||
##Do not open door if red light is flashing, or the pressure gauge is above zero | |||
#Turn off autoclave when finished | |||
Latest revision as of 12:47, 5 July 2012
Back to Protocols
Large Autoclave (Room 435: by media prep room)
- Ask Lab Manager for instruction before using for first time!
- Do not open door during a run. Escaping Steam presents a burn risk!
- If there is an emergency, turn autoclave off and leave it to vent itself!
- Always use log book
- Turn autoclave on
- Select cycle
- Cycle 1: Media less than 1L
- Cycle 2: Media 1L or more
- Cycle 3: Glassware
- Cycle 4: Biological waste
- Put items into autoclave in Nalgene autoclavable trays
- Close door
- Wait for ready light to come on
- Press start button
- Do not open door until cycle is fully complete
- Open door a crack to and let stem vent
- Turn off autoclave when finished
Small Autoclave (Room 409 by RNA room)
- Ask Lab Manager for instruction before using for first time!
- Do not open door during a run. Escaping Steam presents a burn risk!
- If there is an emergency, turn autoclave off and leave it to vent itself!
- Make sure that the internal water level inside the autoclave chamber is even with top of metal platform.
- If it is not fill with dH2O until it is. This will ensure your samples get autoclaved, and not burnt.
- Make sure external reservoir is filled so that the water level is between the high and low mark
- If it is not fill with dH2O or empty so that it is
- Place samples to be autoclaved into the chamber using metal cages to stack multiple samples
- Shut door and turn wheel until door is firmly locked
- Place magnetic key into receptacle
- Turn autoclave on
- Adjust time and temperature
- Press the start button
- Wait till run is completely finished before opening door
- Do not open door if red light is flashing, or the pressure gauge is above zero
- Turn off autoclave when finished
