Library Construction Strain Box 1

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All in MC4100, pSB1A2 unless otherwise specified.

1 ........ ........
11 CW25553 pJAT8 BL21(DE3) pLysS I13401 I13401 (4A2) I13401 (2K3) I13401 (3K3)
21 I13402 (2K3) I13403 (1AK3) I13404 I13405 I13407 I13408
31 I13410 (1AK3) I13412 I13413
51 I0500 (2K3) I6300 (DH5a) I6301 Q04400 (2K3) I15015 I15016 I15017
61 I13404 (DH5a) I13405 (DH5a) I13450 (1A3) I13452 (1A3)
71 I13500 I13502 I13503 I13504 I13505
81
91
Number of Transformations
Reagents 1 5 (6X) 10 (11X)
PEG 3500 50% w/v 240 µl 1440 µl 2640 µl
LiAc 1.0 M 36 µl 216 µl 396 µl
Boiled SS-carrier DNA 50 µl 300 µl 550 µl
Plasmid DNA plus Water 34 µl 204 µl 374 µl
Total 360 µl 2160 µl 3960 µl

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