Lactobacillus planarum miniprep

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Method to miniprep plasmid DNA from Lactobacillus plantarum


  1. Pretreat bacteria with 5mg/ml lysozyme, 15U/ml mutanolysin, 100ug/ml RNase
  2. incubate at 37C for 15-30 minutes
  3. Perform QIAGEN miniprep
  4. Verify plasmid existance by sequencing or running digests on gel


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Relevant papers and books

Mathiesen et al, Journal of Applied Microbiology 2004


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