Difference between revisions of "Koeris/Notebook/2007-1-23"

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(SDS-PAGE)
 
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=Expression assay for [His]<sub>6</sub> constructs=
 
=Expression assay for [His]<sub>6</sub> constructs=
 
I grew the strains overnight at 37 deg C, 300rpm, in 5ml LB + Kan, took 100ul aliquot and froze at -20 deg C.
 
I grew the strains overnight at 37 deg C, 300rpm, in 5ml LB + Kan, took 100ul aliquot and froze at -20 deg C.
==SDS-PAGE==
 
We're using the Invitrogen Nu-PAGE system with Novex bis-tris 4-12% gels' and MES running buffer to get the high resolution in the low kDa-range (as advertised).
 

Latest revision as of 14:36, 24 January 2007

Expression assay for [His]6 constructs

I grew the strains overnight at 37 deg C, 300rpm, in 5ml LB + Kan, took 100ul aliquot and froze at -20 deg C.