Difference between revisions of "Koeris/Notebook/2007-1-21"

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(Pipetting Scheme)
(hipA assay)
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=hipA assay=
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=delta hipA assay=
 
==Growth conditions==
 
==Growth conditions==
 
Grew all samples from previous 16h ON in
 
Grew all samples from previous 16h ON in

Revision as of 09:23, 24 January 2007

delta hipA assay

Growth conditions

Grew all samples from previous 16h ON in

  • 50ml LB + Cam

or

  • 50ml M9 + 0.2% glucose + Cam

for another 16h, at 37 deg C and 300rpm shaking

Persister assay

Pipetting Scheme

No antibiotic No antibiotic No antibiotic No antibiotic Kanamycin 30 ug/ml Kanamycin 30 ug/ml
wt LB wt M9 DhipBA LB DhipBA M9 DhipA LB DhipA M9
Serial dilution Serial dilution Serial dilution Serial dilution Serial dilution Serial dilution