Difference between revisions of "Koch Lab:Protocols/Unzipping constructs/17mer/Anchoring segment"

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This page describes the formation of the "anchoring segment" which is subsequently ligated to the insert oligo to create the base unzipping construct.
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==Materials==
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===Plasmid===
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===Primers===
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===PCR Materials===
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===Restriction digest===
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===Gel extraction===
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==Procedure==
  
 
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Revision as of 20:51, 14 June 2008

Moving pan.gif

This page describes the formation of the "anchoring segment" which is subsequently ligated to the insert oligo to create the base unzipping construct.

Materials

Plasmid

Primers

PCR Materials

Restriction digest

Gel extraction

Procedure