Difference between revisions of "Koch Lab:Protocols/Unzipping constructs/17mer/Adapter duplex"

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==Overview==
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==Materials==
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===Oligonucleotides===
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===Oligo resuspension buffer===
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===Oligo annealing buffer===
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==Procedure==
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===Resuspend Oligos===
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===Anneal duplexes===
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==Storage and handling==
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Store in 0.5 ml annealing tube.  Freeze thaw cycles should not be an issue, but try not to bring too far above 4C when thawing.
  
 
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Revision as of 11:01, 15 June 2008

Moving pan.gif

Overview

Materials

Oligonucleotides

Oligo resuspension buffer

Oligo annealing buffer

Procedure

Resuspend Oligos

Anneal duplexes

Storage and handling

Store in 0.5 ml annealing tube. Freeze thaw cycles should not be an issue, but try not to bring too far above 4C when thawing.