Difference between revisions of "Koch Lab:Inventory/BRB80 (PEM)"

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Line 10: Line 10:
 
**1mM EGTA
 
**1mM EGTA
 
**80mM PIPES
 
**80mM PIPES
**125mM NaOH
+
**12.5mM NaOH
 
+
*New batch made 8/28/2009 [http://www.openwetware.org/wiki/User:Linh_N_Le/Notebook/2009/08/28#PEM]
 +
**80mM PIPES
 +
**1mM EGTA
 +
**1mM MgCl<sub>2</sub>
 +
**12.9mM NaOH
 
===References===
 
===References===
 
* [http://www.cytoskeleton.com/products/buffers/bst01.html Cytoskeleton PEM recipe]
 
* [http://www.cytoskeleton.com/products/buffers/bst01.html Cytoskeleton PEM recipe]

Revision as of 11:19, 28 August 2009

Left Arrow KochLab Inventory.PNG KochLab VWR Fridge
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BRB80 or PEM (PIPES, EGTA, Magnesium) buffer

Desired: 80 mM PIPES, 1 mM EGTA, 1 mM MgCl2, pH 6.9

  • This is the page for the buffer that Andy, Brigette and Linh made on 19:07, 22 June 2009 (EDT)
  • About 205ml were created\
  • Concentrations:
    • 1mM MgCl2
    • 1mM EGTA
    • 80mM PIPES
    • 12.5mM NaOH
  • New batch made 8/28/2009 [1]
    • 80mM PIPES
    • 1mM EGTA
    • 1mM MgCl2
    • 12.9mM NaOH

References