Knight:TOPO TA cloning

Notes

• Tom Knight claims you can cut the reaction volume in half and it will still work fine.
• Use fresh PCR product for best results. (The A overhang tends to get chewed back over time).
• Your primers should NOT have 5' phosphates. They interfere with topoisomerase I.

Resources

• Website
• Manual
• Product notes
• Brochure
• pCR4-TOPO