Knight:TOPO TA cloning

From OpenWetWare
Revision as of 13:30, 29 September 2005 by JeremyZucker (talk | contribs) (Notes)
Jump to: navigation, search


  • Tom Knight claims you can cut the reaction volume in half and it will still work fine.
  • Use fresh PCR product for best results. (The A overhang tends to get chewed back over time).
  • Your primers should NOT have 5' phosphates. They interfere with topoisomerase I.