A lot of useful safety information has been written up here.
Information and training
See Heather's very complete explanation of all the safety training you need to do. The only adjustments for the Knight lab are that you should read the CSAIL chemical hygiene plan (not the Biology one) and you should do the Knight lab specific chemical training. Both of these are linked below.
General safety resources
CSAIL Chemical Hygiene Plan
Once you've read this document, sign the compliance form posted on the bulletin board outside 32-306.
Lab chemical training
Lab chemical training. Template from Kathleen Gilbert, EHS, x3-8409. Modified by Reshma.
Once you've undergone this training, sign the compliance form posted on the bulletin board outside 32-306.
Acetic acid and other organic acids
From Kathleen Gilbert, EHS, x3-8409
Acetic acid is tricky because it is an organic acid that is ignitable and it is more compatible with other organic flammables than with inorganic acids. Acetic acid can be stored with the flammables in a flammable cabinet without a secondary container. If Acetic acid is stored with inorganic acids, it should be in a separate secondary container since it is incompatible with inorganic acids. Trifluoracetic acid is also an organic acid so the same rules apply as with acetic acid.
You do not need the acids stored in a closed [secondary] container. A gray bin is fine.
Equipment validation testing
- Autoclave validation
- Run the eye washes and showers regularly to check that they are working.
Some brief instructions on how to properly dispose of waste in the lab.