Difference between revisions of "Knight:Reconstituting primers"

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(Notes)
 
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===Long version===
 
===Long version===
<math>Y\ \mu L\ =\ \frac{1}{25\ \mu L}*X\ nmoles\ primer\ =\ \frac{1*10^6\ \mu M}{25000\ nmoles}*X\ nmoles\ primer\ =\ 40\ *\ X\ nmoles\ primer</math>
+
<math>Y\ \mu L\ =\ \frac{1 L}{25\ \mu moles}*X\ nmoles\ primer\ =\ \frac{1*10^6\ \mu L}{25000\ nmoles}*X\ nmoles\ primer\ =\ 40\ *\ X\ nmoles\ primer</math>
  
 
==Notes==
 
==Notes==

Latest revision as of 06:12, 7 May 2010

Procedure

Invitrogen recommends the following reconstitution procedure -

  1. Centrifuge the tube for a few seconds to get all the DNA to the bottom of the tube.
  2. To make a 25 μM stock, add YμL of sterile H2O to X nmoles of dry primer stock. (See equations below).
  3. Allow to sit for 2 mins, then vortex for 15 secs.

Short version

Failed to parse (MathML with SVG or PNG fallback (recommended for modern browsers and accessibility tools): Invalid response ("Math extension cannot connect to Restbase.") from server "https://api.formulasearchengine.com/v1/":): {\displaystyle Y\ \mu L\ =\ 40\ *\ X\ nmoles\ primer}

The number of nmoles of material in the tube (X) should be listed on the pages accompanying your primer order.

Long version

Failed to parse (MathML with SVG or PNG fallback (recommended for modern browsers and accessibility tools): Invalid response ("Math extension cannot connect to Restbase.") from server "https://api.formulasearchengine.com/v1/":): {\displaystyle Y\ \mu L\ =\ \frac{1 L}{25\ \mu moles}*X\ nmoles\ primer\ =\ \frac{1*10^6\ \mu L}{25000\ nmoles}*X\ nmoles\ primer\ =\ 40\ *\ X\ nmoles\ primer}

Notes

See Reconstituting primers for a lot of useful information including why you should use TE buffer rather than water for reconstituting primers.