# Difference between revisions of "Knight:Reconstituting primers"

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===Long version=== | ===Long version=== | ||

− | <math>Y\ \mu L\ =\ \frac{1}{25\ \mu | + | <math>Y\ \mu L\ =\ \frac{1 L}{25\ \mu moles}*X\ nmoles\ primer\ =\ \frac{1*10^6\ \mu L}{25000\ nmoles}*X\ nmoles\ primer\ =\ 40\ *\ X\ nmoles\ primer</math> |

==Notes== | ==Notes== |

## Latest revision as of 07:12, 7 May 2010

## Procedure

Invitrogen recommends the following reconstitution procedure -

- Centrifuge the tube for a few seconds to get all the DNA to the bottom of the tube.
- To make a 25 μM stock, add YμL of sterile H
_{2}O to X nmoles of dry primer stock. (See equations below). - Allow to sit for 2 mins, then vortex for 15 secs.

### Short version

[math]Y\ \mu L\ =\ 40\ *\ X\ nmoles\ primer[/math]

The number of nmoles of material in the tube (X) should be listed on the pages accompanying your primer order.

### Long version

[math]Y\ \mu L\ =\ \frac{1 L}{25\ \mu moles}*X\ nmoles\ primer\ =\ \frac{1*10^6\ \mu L}{25000\ nmoles}*X\ nmoles\ primer\ =\ 40\ *\ X\ nmoles\ primer[/math]

## Notes

See Reconstituting primers for a lot of useful information including why you should use TE buffer rather than water for reconstituting primers.