Difference between revisions of "Knight:Reconstituting primers"

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===Long version===
 
===Long version===
<math>Y\ \mu L\ =\ \frac{1}{25\ \mu L}*X\ nmoles\ primer\ =\ \frac{1*10^6\ \mu M}{25000\ nmoles}*X\ nmoles\ primer\ =\ 40\ *\ X\ nmoles\ primer</math>
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<math>Y\ \mu L\ =\ \frac{1 L}{25\ \mu moles}*X\ nmoles\ primer\ =\ \frac{1*10^6\ \mu L}{25000\ nmoles}*X\ nmoles\ primer\ =\ 40\ *\ X\ nmoles\ primer</math>
  
 
==Notes==
 
==Notes==
 
See [[Reconstituting primers]] for a lot of useful information including why you should use TE buffer rather than water for reconstituting primers.
 
See [[Reconstituting primers]] for a lot of useful information including why you should use TE buffer rather than water for reconstituting primers.
  
[[Category:Protocol]] [[Category:DNA]]
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[[Category:Protocol]] [[Category:DNA]] [[Category:In vitro]]

Latest revision as of 06:12, 7 May 2010

Procedure

Invitrogen recommends the following reconstitution procedure -

  1. Centrifuge the tube for a few seconds to get all the DNA to the bottom of the tube.
  2. To make a 25 μM stock, add YμL of sterile H2O to X nmoles of dry primer stock. (See equations below).
  3. Allow to sit for 2 mins, then vortex for 15 secs.

Short version

[math]Y\ \mu L\ =\ 40\ *\ X\ nmoles\ primer[/math]

The number of nmoles of material in the tube (X) should be listed on the pages accompanying your primer order.

Long version

[math]Y\ \mu L\ =\ \frac{1 L}{25\ \mu moles}*X\ nmoles\ primer\ =\ \frac{1*10^6\ \mu L}{25000\ nmoles}*X\ nmoles\ primer\ =\ 40\ *\ X\ nmoles\ primer[/math]

Notes

See Reconstituting primers for a lot of useful information including why you should use TE buffer rather than water for reconstituting primers.