Difference between revisions of "Knight:Purification of His-tagged proteins"

From OpenWetWare
Jump to: navigation, search
Line 8: Line 8:
 
==Protocols==
 
==Protocols==
 
*[[Knight:Purification of His-tagged proteins/Denaturing]]
 
*[[Knight:Purification of His-tagged proteins/Denaturing]]
 +
*[[Knight:Purification of His-tagged proteins/Denaturing with refolding]]
 
*[[Knight:Purification of His-tagged proteins/Native]] <font color=red>(in progress!)</font>
 
*[[Knight:Purification of His-tagged proteins/Native]] <font color=red>(in progress!)</font>
  

Revision as of 07:33, 5 October 2006

<html><style type='text/css'> .tabs {

 width: 750px;
 font-family: trebuchet ms;

}

.tabs strong{

 color: #602;

} </style></html>

CSAIL logo
Knight Lab



Overview

"The high affinity of the Ni-NTA resins for 6xHis-tagged proteins or peptides is due to both the specificity of the interaction between histidine residues and immobilized nickel ions and to the strength with which these ions are held to the NTA resin." [1]

Protocols

References

  1. Qiagen Ni NTA Spin Kit manual [QiagenNTAManual]
  2. Sauer:Purification of His-tagged proteins [SauerPurificationProtocol]