Difference between revisions of "Knight:Protocols"

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[[Knight:DNA ligation using NEB Quick Ligation Kit]]
[[Knight:DNA ligation using NEB Quick Ligation Kit]]
[[Knight:Micropure EZ and Microcon purification]]
[[Knight:Micropure EZ and Microcon purification]]

Revision as of 13:18, 10 December 2006

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CSAIL logo
Knight Lab


Knight:Agarose gel electrophoresis

Annealing and primer extension

Knight:Annealing and primer extension with Taq polymerase

Knight:Colony PCR

Ethanol precipitation of small DNA fragments

Knight:DNA ligation using NEB Quick Ligation Kit


Knight:Micropure EZ and Microcon purification


Phosphatase treatment of linearized vector

Knight:Reconstituting primers

Knight:Restriction Digest

Knight:Sequencing DNA

Knight:TOPO TA cloning

Knight:Addition of 3' A-overhangs

Knight:Site-directed mutagenesis


Knight:In vitro transcription --in progress!

Knight:RNA electrophoresis --in progress!


Knight:Protein solubility

Knight:Centrifuge desalting

Knight:Dialysis --works poorly

Knight:Electrophoretic mobility shift assay

Knight:Gel filtration

Knight:Microcon filtration of proteins --works poorly

Knight:NuPAGE electrophoresis

Knight:Protein DNA binding

Knight:Purification of His-tagged proteins

Escherichia coli

Preparing chemically competent cells (Inoue)

Transforming chemically competent cells (Inoue)

Knight:Preparing electrocompetent cells




Calibrating the pH meter

Measuring pH

Knight:Poster printing