Difference between revisions of "Knight:Protocols"

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[[Knight:Site-directed mutagenesis]]
 
[[Knight:Site-directed mutagenesis]]
 +
 +
==RNA==
 +
[[Knight:In vitro transcription]] --<font color=red>in progress!</font>
  
 
==Protein==
 
==Protein==
[[Knight:Protein solubility]] --<font color=red>in progress!</font>
+
[[Knight:Protein solubility]]
  
 
[[Knight:Centrifuge desalting]]
 
[[Knight:Centrifuge desalting]]
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[[Knight:Dialysis]] --<font color=red>works poorly</font>
 
[[Knight:Dialysis]] --<font color=red>works poorly</font>
  
[[Knight:Electrophoretic mobility shift assay]] --<font color=red>in progress!</font>
+
[[Knight:Electrophoretic mobility shift assay]]
  
[[Knight:Gel filtration]] --<font color=red>in progress!</font>
+
[[Knight:Gel filtration]]
  
 
[[Knight:Microcon filtration of proteins]] --<font color=red>works poorly</font>
 
[[Knight:Microcon filtration of proteins]] --<font color=red>works poorly</font>
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[[Knight:NuPAGE electrophoresis]]  
 
[[Knight:NuPAGE electrophoresis]]  
  
[[Knight:Protein DNA binding]] --<font color=red>in progress!</font>
+
[[Knight:Protein DNA binding]]
  
 
[[Knight:Purification of His-tagged proteins]]
 
[[Knight:Purification of His-tagged proteins]]

Revision as of 09:10, 28 November 2006

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CSAIL logo
Knight Lab



DNA

Knight:Agarose gel electrophoresis

Annealing and primer extension

Knight:Annealing and primer extension with Taq polymerase

Knight:Colony PCR

Ethanol precipitation of small DNA fragments

Knight:DNA ligation using NEB Quick Ligation Kit

Knight:Micropure EZ and Microcon purification

Miniprep

Phosphatase treatment of linearized vector

Knight:Reconstituting primers

Knight:Restriction Digest

Knight:Sequencing DNA

Knight:TOPO TA cloning

Knight:Addition of 3' A-overhangs

Knight:Site-directed mutagenesis

RNA

Knight:In vitro transcription --in progress!

Protein

Knight:Protein solubility

Knight:Centrifuge desalting

Knight:Dialysis --works poorly

Knight:Electrophoretic mobility shift assay

Knight:Gel filtration

Knight:Microcon filtration of proteins --works poorly

Knight:NuPAGE electrophoresis

Knight:Protein DNA binding

Knight:Purification of His-tagged proteins

Escherichia coli

Preparing chemically competent cells (Inoue)

Transforming chemically competent cells (Inoue)

Knight:Preparing electrocompetent cells

Knight:Electroporation

Miscellaneous

Knight:Dishwashing

Calibrating the pH meter

Measuring pH

Knight:Poster printing