Knight:Protocols: Difference between revisions
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[[Knight:Site-directed mutagenesis]] | [[Knight:Site-directed mutagenesis]] | ||
==RNA== | |||
[[Knight:In vitro transcription]] --<font color=red>in progress!</font> | |||
==Protein== | ==Protein== | ||
[[Knight:Protein solubility]] | [[Knight:Protein solubility]] | ||
[[Knight:Centrifuge desalting]] | [[Knight:Centrifuge desalting]] | ||
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[[Knight:Dialysis]] --<font color=red>works poorly</font> | [[Knight:Dialysis]] --<font color=red>works poorly</font> | ||
[[Knight:Electrophoretic mobility shift assay]] | [[Knight:Electrophoretic mobility shift assay]] | ||
[[Knight:Gel filtration]] | [[Knight:Gel filtration]] | ||
[[Knight:Microcon filtration of proteins]] --<font color=red>works poorly</font> | [[Knight:Microcon filtration of proteins]] --<font color=red>works poorly</font> | ||
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[[Knight:NuPAGE electrophoresis]] | [[Knight:NuPAGE electrophoresis]] | ||
[[Knight:Protein DNA binding]] | [[Knight:Protein DNA binding]] | ||
[[Knight:Purification of His-tagged proteins]] | [[Knight:Purification of His-tagged proteins]] | ||
Revision as of 10:10, 28 November 2006
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Knight Lab
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DNA
Knight:Agarose gel electrophoresis
Annealing and primer extension
Knight:Annealing and primer extension with Taq polymerase
Ethanol precipitation of small DNA fragments
Knight:DNA ligation using NEB Quick Ligation Kit
Knight:Micropure EZ and Microcon purification
Phosphatase treatment of linearized vector
Knight:Addition of 3' A-overhangs
Knight:Site-directed mutagenesis
RNA
Knight:In vitro transcription --in progress!
Protein
Knight:Dialysis --works poorly
Knight:Electrophoretic mobility shift assay
Knight:Microcon filtration of proteins --works poorly
Knight:Purification of His-tagged proteins
Escherichia coli
Preparing chemically competent cells (Inoue)
Transforming chemically competent cells (Inoue)
Knight:Preparing electrocompetent cells
