Difference between revisions of "Knight:Protocols"

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==Protein==
 
==Protein==
[[Knight:Purification of His-tagged proteins]] -- <font color=red>in progress!</font>
+
[[Knight:Purification of His-tagged proteins]]
  
[[Knight:NuPAGE electrophoresis]] --<font color=red>in progress!</font>
+
[[Knight:NuPAGE electrophoresis]]
 +
 
 +
[[Knight:Electrophoretic mobility shift assay]] --<font color=red>in progress!</font>
  
 
==''Escherichia coli''==
 
==''Escherichia coli''==

Revision as of 07:32, 25 August 2006

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CSAIL logo
Knight Lab



DNA

Knight:Agarose gel electrophoresis

Annealing and primer extension

Knight:Annealing and primer extension with Taq polymerase

Knight:Colony PCR

Ethanol precipitation of small DNA fragments

Knight:DNA ligation using NEB Quick Ligation Kit

Micropure EZ and Microcon purification

Miniprep

Phosphatase treatment of linearized vector

Knight:Reconstituting primers

Knight:Restriction Digest

Knight:Sequencing DNA

Knight:TOPO TA cloning

Knight:Addition of 3' A-overhangs

Knight:Site-directed mutagenesis

Protein

Knight:Purification of His-tagged proteins

Knight:NuPAGE electrophoresis

Knight:Electrophoretic mobility shift assay --in progress!

Escherichia coli

Preparing chemically competent cells (Inoue)

Transforming chemically competent cells (Inoue)

Knight:Preparing electrocompetent cells

Knight:Electroporation

Miscellaneous

Calibrating the pH meter

Measuring pH

Knight:Poster printing