Difference between revisions of "Knight:Protocols"

From OpenWetWare
Jump to: navigation, search
Line 35: Line 35:
[[Knight:Purification of His-tagged proteins]] -- <font color=red>in progress!</font>
[[Knight:Purification of His-tagged proteins]]
[[Knight:NuPAGE electrophoresis]] --<font color=red>in progress!</font>
[[Knight:NuPAGE electrophoresis]]
[[Knight:Electrophoretic mobility shift assay]] --<font color=red>in progress!</font>
==''Escherichia coli''==
==''Escherichia coli''==

Revision as of 07:32, 25 August 2006

<html><style type='text/css'> .tabs {

 width: 750px;
 font-family: trebuchet ms;


.tabs strong{

 color: #602;

} </style></html>

CSAIL logo
Knight Lab


Knight:Agarose gel electrophoresis

Annealing and primer extension

Knight:Annealing and primer extension with Taq polymerase

Knight:Colony PCR

Ethanol precipitation of small DNA fragments

Knight:DNA ligation using NEB Quick Ligation Kit

Micropure EZ and Microcon purification


Phosphatase treatment of linearized vector

Knight:Reconstituting primers

Knight:Restriction Digest

Knight:Sequencing DNA

Knight:TOPO TA cloning

Knight:Addition of 3' A-overhangs

Knight:Site-directed mutagenesis


Knight:Purification of His-tagged proteins

Knight:NuPAGE electrophoresis

Knight:Electrophoretic mobility shift assay --in progress!

Escherichia coli

Preparing chemically competent cells (Inoue)

Transforming chemically competent cells (Inoue)

Knight:Preparing electrocompetent cells



Calibrating the pH meter

Measuring pH

Knight:Poster printing