Difference between revisions of "Knight:Protocols"

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[[Knight:Preparing electrocompetent cells]]
 
[[Knight:Preparing electrocompetent cells]]
  
[[Electroporation]]
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[[Knight:Electroporation]]
  
 
==Miscellaneous==
 
==Miscellaneous==

Revision as of 13:11, 18 July 2006

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CSAIL logo
Knight Lab



DNA

Knight:Agarose gel electrophoresis

Annealing and primer extension

Knight:Annealing and primer extension with Taq polymerase

Knight:Colony PCR

Ethanol precipitation of small DNA fragments

Knight:DNA ligation using NEB Quick Ligation Kit

Micropure EZ and Microcon purification

Miniprep

Phosphatase treatment of linearized vector

Knight:Reconstituting primers

Knight:Restriction Digest

Knight:Sequencing DNA

Knight:TOPO TA cloning

Knight:Addition of 3' A-overhangs

Knight:Site-directed mutagenesis

Protein

Knight:Purification of His-tagged proteins -- in progress!

Knight:NuPAGE electrophoresis --in progress!

Escherichia coli

Preparing chemically competent cells (Inoue)

Transforming chemically competent cells (Inoue)

Knight:Preparing electrocompetent cells

Knight:Electroporation

Miscellaneous

Knight:Poster printing