Knight:Protocols: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
(→DNA) |
(→DNA) |
||
Line 21: | Line 21: | ||
[[Phosphatase treatment of linearized vector]] | [[Phosphatase treatment of linearized vector]] | ||
[[Knight:Reconstituting primers]] | |||
[[Knight:Restriction Digest]] | [[Knight:Restriction Digest]] |
Revision as of 12:38, 26 May 2006
<html><style type='text/css'> .tabs {
width: 750px; font-family: trebuchet ms;
}
.tabs strong{
color: #602;
} </style></html>
DNA
Knight:Agarose gel electrophoresis
Annealing and primer extension
Knight:Annealing and primer extension with Taq polymerase
Ethanol precipitation of small DNA fragments
Knight:DNA ligation using NEB Quick Ligation Kit
Micropure EZ and Microcon purification
Phosphatase treatment of linearized vector
Knight:Addition of 3' A-overhangs
Knight:Site-directed mutagenesis
Escherichia coli
Knight:Preparing electrocompetent cells