Difference between revisions of "Knight:Protocols"

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[[Knight:Agarose gel electrophoresis]]
[[Knight:Agarose gel electrophoresis]]
[[Knight:Annealing and cloning oligos]] --<font color=red>in progress, hasn't been tested</font>
[[Annealing and primer extension]]
[[Annealing and primer extension]]
[[Knight:Annealing and primer extension with Klenow polymerase]]
[[Knight:Annealing and primer extension with Taq polymerase]]
[[Knight:Annealing and primer extension with Taq polymerase]]

Revision as of 06:43, 13 July 2007

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CSAIL logo
Knight Lab


Knight:Agarose gel electrophoresis

Knight:Annealing and cloning oligos --in progress, hasn't been tested

Annealing and primer extension

Knight:Annealing and primer extension with Klenow polymerase

Knight:Annealing and primer extension with Taq polymerase

Knight:Colony PCR

Ethanol precipitation of small DNA fragments

Knight:DNA ligation using NEB Quick Ligation Kit


Knight:Micropure EZ and Microcon purification


Phosphatase treatment of linearized vector

Knight:Reconstituting primers

Knight:Restriction Digest

Knight:Sequencing DNA


Knight:TOPO TA cloning

Knight:TOPO vector preparation

Knight:Addition of 3' A-overhangs

Knight:Site-directed mutagenesis


Knight:In vitro transcription

Knight:RNA electrophoresis


Knight:Protein solubility

Knight:Centrifuge desalting

Knight:Dialysis --works poorly

Knight:Electrophoretic mobility shift assay

Knight:Gel filtration

Knight:Microcon filtration of proteins --works poorly

Knight:NuPAGE electrophoresis

Knight:Protein DNA binding

Knight:Purification of His-tagged proteins

Knight:Purification of MBP-tagged proteins

Escherichia coli

Preparing chemically competent cells (Inoue)

Transforming chemically competent cells (Inoue)

TOP10 chemically competent cells

Knight:Preparing electrocompetent cells





Calibrating the pH meter

Measuring pH

Knight:Poster printing