Knight:NuPAGE electrophoresis

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In progress! For a better version of this protocol, see Sauer:bis-Tris SDS-PAGE, the very best.

Purpose

To run a protein gel. Note that a preferable version of this protocol is available at Sauer:bis-Tris SDS-PAGE, the very best (recommended by Kathleen). But since we seem to already have the Invitrogen kit contents in lab, this protocol describes use of the Invitrogen system.

Materials

Procedure

References

  1. NuPAGE Technical Guide [NuPAGETechnicalGuide]