Knight:Materials: Difference between revisions
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*ordered by [[Austin Che | Austin]] (50μM) | *ordered by [[Austin Che | Austin]] (50μM) | ||
==Yeast== | |||
*[[Knight:yeast plasmids|Plasmids]] | |||
*[[Knight:yeast strains|Strains]] | |||
==Dry ice== | ==Dry ice== |
Revision as of 12:51, 29 August 2006
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Common primers
VF2
5' tgc cac ctg acg tct aag aa 3'
VR
5' ATT ACC GCC TTT GAG TGA GC 3'
BioBricks-f
5' GTT TCT TCG AAT TCG CGG CCG CTT CTA G 3'
- ordered by Reshma 5/1/2006 (25μM)
BioBricks-r
5' GTT TCT TCC TGC AGC GGC CGC TAC TAG TA 3'
- ordered by Reshma 8/24/2006
M13-R
5' cag gaa aca gct atg ac 3'
- ordered by Austin (50μM)
M13-F (-20)
5' GTA AAA CGA CGG CCA G 3'
- ordered by Austin (50μM)
Yeast
Dry ice
Dry ice is delivered on Tuesday mornings (before noon). The stash usually lasts all week but is gone some time over the weekend. There's not any remaining by Monday morning.