Difference between revisions of "Knight:Materials"

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==Dry ice==
 
 
Dry ice is delivered on Tuesday mornings (before noon).  The stash usually lasts all week but is gone some time over the weekend.  There's not any remaining by Monday morning.
 
 
==DNA==
 
===Primers===
 
See [[Knight:Primers]].
 
 
===Electrophoresis===
 
*[[Knight:Loading dye]]
 
 
==RNA==
 
*[[Knight:RNA work]]
 
 
==Protein==
 
*[http://www.piercenet.com/products/browse.cfm?fldID=02020105 Coomassie (Bradford) Protein Assay Kit] from Pierce
 
*[http://www.piercenet.com/products/browse.cfm?fldID=02020108 Albumin standard] from Pierce
 
*[http://www.invitrogen.com/content/sfs/manuals/invisionstain_man.pdf InVision™ His-tag In-gel Stain] from Invitrogen (purchased in 2003?)
 
 
===Quartz cuvettes for CD===
 
For our old spectrophotometer, we had two macrocell QS quartz cuvettes and two microcell QS quartz cuvettes.  I tested the quality of these four cuvettes for the purposes of CD (circular dichroism) using the [http://csbi.mit.edu/biophysinstrument/cdspectrometer CD spectrometer] at the [http://csbi.mit.edu/biophysinstrument/ Bioinstrumentation facility].  The two microcell cuvettes were not suitable for CD ... they have high birefringence.  The two macrocell cuvettes were suitable for CD in the wavelenth range 224-320nm.  However, for protein structures like alpha helices, you'll likely want to check the spectra even at 200nm.  So these cuvettes are not great for this purpose.  A cuvette for use in CD was purchased.
 
 
==Stocks==
 
*[[Knight:Dyes]]
 
*[[Knight:Freezer]]
 
*[[Knight:Fridge]]
 
*[[Knight:yeast plasmids|Plasmids]]
 
*[[Knight:yeast strains|Strains]]
 
 
 
 
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Revision as of 20:48, 14 July 2009