Knight:Electrophoretic mobility shift assay

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In progress! Has not been tested.


An assay to check for protein-DNA binding.




  1. EMSA kit from Invitrogen [Invitrogen]
  2. Jing D, Beechem JM, and Patton WF. The utility of a two-color fluorescence electrophoretic mobility shift assay procedure for the analysis of DNA replication complexes. Electrophoresis. 2004 Aug;25(15):2439-46. DOI:10.1002/elps.200405994 | PubMed ID:15300760 | HubMed [Jing-Electrophoresis-2004]
  3. Jing D, Agnew J, Patton WF, Hendrickson J, and Beechem JM. A sensitive two-color electrophoretic mobility shift assay for detecting both nucleic acids and protein in gels. Proteomics. 2003 Jul;3(7):1172-80. DOI:10.1002/pmic.200300438 | PubMed ID:12872218 | HubMed [Jing-Proteomics-2003]
All Medline abstracts: PubMed | HubMed