Klapperich Lab:Notebook/Lab Meeting Notes/2009/10/20: Difference between revisions

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† SPE Column Optimization for RNA. (Lead: Sonali, Team: Hussam, Jessie, Brendan) <br>
† SPE Column Optimization for RNA. (Lead: Sonali, Team: Hussam, Jessie, Brendan) <br>
* Design three straight channels. A: std channel we already use. B: widest channel possible, C: something in between. Deliver CAD drawings for these for photomasks by Friday 10/16, functional chips to be delivered by 10/26 [Hussam/Jessie]
* Design three straight channels. A: std channel we already use. B: widest channel possible, C: something in between. functional chips to be delivered by 10/21-22, Experimental results of channel to be delivered 10/27  [Hussam/Jessie]
* Silica free SPE, and SPE free channel experiment to be repeated. Control was old didn't take pressure, report back on 10/15.<br>
* Load DNA in a straw of 5,50,100ul SPE, quick test of hypothesis: more SPE = more DNA, <i>To be repeated with Alex and Mark </i> <br>
* Load DNA in a straw of 50ul SPE, quick test of hypothesis more SPE = more DNA, report back 10/16. <br>
* New virus prep (JC, 6/11/09), getting Ct's of 27 for 1:16 dilution and 28 for 1:64 dilution.  Use more diluted sample for future optimization experiments?<br>
* New virus prep (JC, 6/11/09), getting Ct's of 27 for 1:16 dilution and 28 for 1:64 dilution.  Use more diluted sample for future optimization experiments?<br>
* Tried 2 more chips with 3X 0.15um Silica.  SPE was coming out even at 2ml/hr flow rate.<br>
* Tried 2 more chips with 3X 0.15um Silica.  SPE was coming out even at 2ml/hr flow rate.<br>

Revision as of 09:54, 20 October 2009

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20 October 2009 Lab Meeting

  • Attending:
  • Missing:
  • Presentation:Dr. Tushar Bansal, post doc applicant. 2pm, please BE ON TIME.


‡ Announcements


  • PAPERS Drafted before MicroTAS
  • Posters for MicroTAS


‡ Flu R01:Integration

  • New design - address evaporation loss, Reagent delivery and storage solution. Consider marginating flows?


† Sample Concentration (Lead: Jane, Team: Jaephil)

  • Up to high 10^7 for positive control. Silver substrate no signal.
  • Jane working on the cell lysate control: plan to have Cassidy's help on plaque assay while I'm away.
  • New cell passage from frozen - the old one is beyond passage 30.
  • Main loss is at the outlet/sample collection: tangential filtration design in drawing stage.
  • Contacted EC Shaw about rubber stamp mold for new design for evaporation.
  • Set up COMSOL and StarCD, look for governing equations for simulation of evaporation.
  • Committee meeting setup


† SPE Column Optimization for RNA. (Lead: Sonali, Team: Hussam, Jessie, Brendan)

  • Design three straight channels. A: std channel we already use. B: widest channel possible, C: something in between. functional chips to be delivered by 10/21-22, Experimental results of channel to be delivered 10/27 [Hussam/Jessie]
  • Load DNA in a straw of 5,50,100ul SPE, quick test of hypothesis: more SPE = more DNA, To be repeated with Alex and Mark
  • New virus prep (JC, 6/11/09), getting Ct's of 27 for 1:16 dilution and 28 for 1:64 dilution. Use more diluted sample for future optimization experiments?
  • Tried 2 more chips with 3X 0.15um Silica. SPE was coming out even at 2ml/hr flow rate.
  • Running no silica and no SPE chips with virus.


† PCR - CMI (Lead: Qingqing)

  • QQ will work on the initial integration steps of SPE + RT (resevoir)+ PCR.
  • PCR2 Paper formatted for LOAC this week.
  • CMK: PCR 1 draft. Analytical Chem. MCK is running more simulations.


† HDA (Lead: Jaephil, Team:Sonali)

  • Start planning R01 for Submission on 2/5/10. MM, JD, CMK.
  • JD will train QQ how to make HDA chips. This may entail moving from cutter plotter to embossing steps. JD and QQ to meet ASAP to plan the tech transfer.
  • Paper submitted 10/6.


‡C. diff Project (Cathie, Sonali, Satish Singh, Lisa J., His post doc )

  • Sonali to train Lisa on SPE.
  • QQ to run test PCR on chip with genomic DNA and Toxin B primers.


‡ Coulter Flu Fraunhofer Project (Lead: Sonali, Team: Sonali, Jessie, Cathie, CMI Folks, Qingqing)

  • Meeting 9am Monday (every other week)


‡ Agilent Automated Sample Preparation (Lead: Alex)

  • Paul finishes work on nozzles on Wednesday. Then first testrun and rewriting of software for "HotDog"-Run on Machine.
  • First Draft of Paper (Lysis of Yeast Cells) ready. Work ongoing on "Conclusion"-Section of Paper.


‡ COBRA (Lead: Jaephil, Team: Cathie, Jane for virus only, PHO folks)

  • Metal piece modification for the integration - Parts distored and is begin fixed.
  • paper 1: Evap with Sol Gel substrate. Not integrated. MSSA, E coli.


‡ Biointerfaces group (Lead: MinCheol, Team: Cathie, MCK, Wong and Meller folks)

  • New experiments happening now.
  • Cathie will submit paper inquiry.


‡ CIMIT- Sepsis (Lead:Cathie, Team:TBA)

  • IRB approved.
  • Cathie submitted the companion BU IRB form for exemption. Revised, still still waiting.


‡ PATH Grant (Lead:Cathie, Team:Frank, Sean, Mark, Jake Trueb (ME), Suma)

  • Phone meeting with PATH this week. 10/13.
  • UGs purchasing parts for straw machine for 709.
  • UGs purchasing reagents for running extraction experiments and doing PCR.




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