Klapperich Lab:Notebook/Lab Meeting Notes/2009/08/25: Difference between revisions
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† Flu R01 <br> | † Flu R01 <br> | ||
* Do std curve without carrier RNA. Compare with ASB data. <br> | * Do std curve without carrier RNA. Compare with ASB data. <br> | ||
* Integration: Spec? On chip? Jane will look into it. <br> | * Integration: Spec? On chip? Jane will look into it. <br>''' | ||
Are we still looking to do this on chip? For on chip, we need to fabricate mirrors such that light can access assays in channels. For off chip, we need to look into OEM light source, fiber optices, fluorescence spectrometer, and detectors. They are pricey. such as this one: http://www.oceanoptics.com/products/usb4000fl.asp''' <br> | |||
* QQ will work on the initial integration steps of SPE + RT (resevoir)+ PCR. Cutter plotter? Faunhofer Chip serpentine channel. "QQ Needs to know how to make valves." <br> | * QQ will work on the initial integration steps of SPE + RT (resevoir)+ PCR. Cutter plotter? Faunhofer Chip serpentine channel. "QQ Needs to know how to make valves." <br> | ||
* RNA extraction troubleshooting. <br> | * RNA extraction troubleshooting. <br> | ||
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† Virus concentration (upstream from the assay.) <br> | † Virus concentration (upstream from the assay.) <br> | ||
* How to read? Color, sensitivity? Alignment. Check with JD. <br> | * How to read? Color, sensitivity? Alignment. Check with JD. <br> | ||
<br> | '''Alexa Fluo-488 succinimidyl ester (Molecular Probes) stains the flu virus fluorescent. <br> | ||
'''The problem is not how to visualize virus right now, because the design is clearly not optimized for collecting all the samples are the outlet. The major loss is from sample handling instead of from non specific binding to the material.''' | |||
† Coulter Flu Fraunhofer Project | † Coulter Flu Fraunhofer Project | ||
* IBC for Fraun flu. approved. <br> | * IBC for Fraun flu. approved. <br> |
Revision as of 00:13, 25 August 2009
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25 August 2009 Lab Meeting
† Flu R01
Are we still looking to do this on chip? For on chip, we need to fabricate mirrors such that light can access assays in channels. For off chip, we need to look into OEM light source, fiber optices, fluorescence spectrometer, and detectors. They are pricey. such as this one: http://www.oceanoptics.com/products/usb4000fl.asp
* Need to update IBC to include rDNA work. † Virus concentration (upstream from the assay.)
Alexa Fluo-488 succinimidyl ester (Molecular Probes) stains the flu virus fluorescent. † Coulter Flu Fraunhofer Project
† COBRA
paper 1: Evap with Sol Gel substrate.
* on-chip PCR on a series of diluted lambda phage DNA. the detect limitation for ABI is 10-9g/ul on-chip PCR have the same sensitivity. * With new syring and tubing, on-chip PCR for the patient sample does not have detectable amplified product. however, the postitive control on the thermal cycle works good. † RCA/HDA
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