Difference between revisions of "Klapperich Lab:Notebook/Lab Meeting Notes/2009/06/09"

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(9 June 2009 Lab Meeting)
(9 June 2009 Lab Meeting)
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- Signals are similar between with and without GNP <br>
- Signals are similar between with and without GNP <br>
- Rhodamine binds poorly to gold <br>
- Rhodamine binds poorly to gold <br>
- silver NP?
- try GNP-pMA next. R6G-silver NP.
- Need to redefine hypothesis
- Need to redefine hypothesis

Revision as of 07:07, 9 June 2009

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9 June 2009 Lab Meeting

† Announcements

  • Short small team meetings. 45 min -- SEE GOOGLE calendar for schedule

Suggested groups... please edit. Teams
2. Bacteria = JZ, JD
3. Flu = MM, JZ, HM, JC, BC
3. Sample Preparation = AG, HM?

  • MicroTAS Abstracts accepted. (4) Papers due June 30th.

† Flu R01

  • RNA extraction troubleshooting.
  • Absolute Quantification assays: TH this week.
  • FTIR

† Coulter Flu Fraunhofer Project

  • Fraun folks coming to get trained next week. RNA isolation.
  • IBC for Fraun flu. approved?

† SEPSIS Project

  • Accepted YEAH!

†RNA project

  • Call Natalia.


  • Test with sol-gel substrate today(2nd June)
  • delayed due to difficulty adjusting membrane thickness
  • Evap system (JD and JZ)

    • Let's get this to a point where we can publish and move on

paper 1: Evap with Sol Gel substrate.
paper 2: Covap with PMA, Rhodamine, PS beads?...virus...?
- Rhodamine experiments done
- Signals are similar between with and without GNP
- Rhodamine binds poorly to gold
- try GNP-pMA next. R6G-silver NP. - Need to redefine hypothesis
† Virus concentration

† Valve Array

  • Teddy will give training presentation(guide).

† Fraunhofer: LOAC

  • Paper revised.
  • Bonding ongoing with MIT folks

† Biointerfaces group

  • Meeting Wed 2-4pm.
  • fabricated SU-8 mold with Herringbone grooves.
  • Brett will take a photo of the device for the paper.
  • Jason coated thin gold on the surface of the PDMS mold, and will take SEM images for the sieves.
  • draft paper2 will be started soon.

† CIMIT- Colson Grant

  • IRB still in revision.


  • PCR 2 paper draft.
  • PEG-coating protocol developed
  • on-chip experiment with blank chip without PEG,BSA. It works with expected lower efficiency
  • on-chip experiment with PEG-coated chip without PEG,BSA in reagent. It does not work, or the product is under the detection limitation of bioanalyzer.


  • Getting solution back out is still an issue.
  • Integrated chip worked (19th). Accumulating multiple runs. (Check into primer lifetime)
  • Evaporation problems near edges. Maybe design change?
  • Teflon issue with the enzyme? Check into it.

† Silica Optimization (Lambda):

  • Get lower Bioan. Kit.