Difference between revisions of "Klapperich Lab:Notebook/Lab Meeting Notes/2008/09/30"

From OpenWetWare
Jump to: navigation, search
(30 September 2008 Lab Meeting Agenda)
(30 September 2008 Lab Meeting Agenda)
Line 81: Line 81:
† Silica Optimization (Lambda): <br>
† Silica Optimization (Lambda): <br>
* Ongoing <br>
* Ongoing <br>
[http://openwetware.org/wiki/Image:Progress_9-22_to_9-28.doc hussam's update] <br>
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Revision as of 07:06, 30 September 2008

Owwnotebook icon.png Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

30 September 2008 Lab Meeting Agenda

† No research report today.

† Announcements

  • A slow migration to 709 can now begin. There will be core equipment entering 720, we will accommodate.
  • Supply costs are out of control! Conserve.

† Flu R01

  • Mark's role outlined this week. He will make fixture for Sonali in near term. Will look at integrated chip in long term.
  • Will do RTPCR and not anti sera assays - Cathie told Chris et al. 9/29.
  • More sample back? Can we put in 1.5 ml saline, collect in 3ml VTM tube directly? Can we amend - Cathie emailed on 9/29.
  • Plaque assay status?
  • Cathie STILL has to submit the progress report to INSPIR.

† SEPSIS Project - CIMIT Bird Flu

  • Toluene method. Why didn't we use it? (QQ, AG)
  • Sample (raw pellets and film) from Ticona COC ordered.
  • B Sub data in blood extracted redo PCR/contaminated water.
  • PCR Pipettes bought...no one uses them for non-PCR use!!!!!!!!
  • E coli in pee/blood. This week.
  • SEM image analysis of pore size. Jane did preliminary analysis. Can Jane finish this up for the paper?

†RNA project

  • Sonali will teach Stephanie Jurkat Culture.
  • Jeff Braman will visit in early Dec.
  • We have Anirban's protocol and system etc. Issues resolved


  • Mark finish control circuit (Temp, humidity, pressure) design and ordered parts
  • Fluorescence microscopy available from 720. Try beads experiment.
  • Cutter plotter ordered to make new fluidic layer
  • Jane, Cathie, Larry, Ranjith will meet soon to discuss scale up of sol gel in chip process.
  • Current design gives E.coli signal, clotting is still a problem
  • Change of design: funnel-shaped wells, smooth hole, make samples in pipette tips?
  • Jane working on CIMIT fellowship presentation and future plans for THESIS!

† Valve Array/Valve building (FJ)

  • Waiting for fixture still from Melissa
  • Update: Monday 29 Sept Frank contacted Shane Conran of Superior Fluid Solutions (referred by Jess from UCSB). Will draw up preliminary design and send to us for evaluation.

† Fraunhofer: LOAC

  • Still Still Still troubleshooting ??

† Biointerfaces group

  • Make a mold for Brett this week.
  • Do preliminary E. coli experiment soon.
  • Making a CR mask Tuesday afternoon.

† CIMIT- Colson Grant

  • Preliminary plan for IRB submission at Harvard in place. Target date 1/1/09
  • STAR-CD Renewal - January ($2K)


  • PCR areas defined. Protocols reviewed. Contamination issues discussed....
  • QQ designed a TaqMan Assay for the lambda phage PCR
  • Contamination. Make an internal control on the chip.
  • What is the time consuming step on using a new chip every time? (QQ)
  • MinCheol is making Four simple PCR models to optimize temperature distribution along to the channel.
  • MinCheol is simulating DNA's Residence Time for simple PCR models.


  • Cathie Still Working on Whitepaper almost done!

† Senior project

† F31: Cochlea

  • ARO Abstract due October 1st.

† Silica Optimization (Lambda):

  • Ongoing

hussam's update