Difference between revisions of "Ketner Lab"

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(Antibodies)
(Protocols)
Line 20: Line 20:
 
*Calculate MOI
 
*Calculate MOI
 
*Protein preps of infected 293 cells for westerns
 
*Protein preps of infected 293 cells for westerns
*[[Western Blots]]
+
*[http://www.openwetware.org/index.php?title=Ketner:_Tissue_culture_viral_lysate_Western_Blot Western Blot]
 
*P32 label DNA
 
*P32 label DNA
  

Revision as of 10:01, 6 September 2006

JHSPHbanner.jpg


Current Projects

Adenovirus Vaccines

Ubiquination


Protocols

  • Adenovirus CaCl transfection
  • Generating hexon mutants in E. coli
  • Generating fiber mutants in E. coli
  • Generating late gene expression mutants in 293 cells
  • Purifying adenovius (banded stocks)
  • Titration of viral stocks (calculate pfu)
  • Calculate MOI
  • Protein preps of infected 293 cells for westerns
  • Western Blot
  • P32 label DNA

Materials

Antibodies

Links

Gary Ketner

JHSPH Magazine