Difference between revisions of "Ketner Lab"

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(Antibodies)
Line 25: Line 25:
 
==Materials==
 
==Materials==
 
===Antibodies===
 
===Antibodies===
*anti-HA
+
*[[anti-HA]]
*anti-Fiber
+
*[[anti-Fiber]]
*anti-Late serum
+
*[[anti-Late serum]]
*anti-NANP hybridoma supernatant
+
*[[anti-NANP hybridoma supernatant]]
  
 
== Links ==
 
== Links ==

Revision as of 09:19, 6 September 2006

JHSPHbanner.jpg


Current Projects

Adenovirus Vaccines

Ubiquination


Protocols

  • Adenovirus CaCl transfection
  • Generating hexon mutants in E. coli
  • Generating fiber mutants in E. coli
  • Generating late gene expression mutants in 293 cells
  • Purifying adenovius (banded stocks)
  • Titration of viral stocks (calculate pfu)
  • Calculate MOI
  • Protein preps of infected 293 cells for westerns
  • Western Blots
  • P32 label DNA

Materials

Antibodies

Links

Gary Ketner

JHSPH Magazine