Difference between revisions of "Ketner Lab"

From OpenWetWare
(Protocols)
Line 20: Line 20:
 
*Calculate MOI
 
*Calculate MOI
 
*Protein preps of infected 293 cells for westerns
 
*Protein preps of infected 293 cells for westerns
*Western Blots
+
*[[Western Blots]]
 
*P32 label DNA
 
*P32 label DNA
 
  
 
== Links ==
 
== Links ==

Revision as of 09:00, 31 August 2006

JHSPHbanner.jpg


Current Projects

Adenovirus Vaccines

Ubiquination


Protocols

  • Adenovirus CaCl transfection
  • Generating hexon mutants in E. coli
  • Generating fiber mutants in E. coli
  • Generating late gene expression mutants in 293 cells
  • Purifying adenovius (banded stocks)
  • Titration of viral stocks (calculate pfu)
  • Calculate MOI
  • Protein preps of infected 293 cells for westerns
  • Western Blots
  • P32 label DNA

Links

Gary Ketner

JHSPH Magazine