Difference between revisions of "Ketner Lab"

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(Protocols)
(Materials)
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*[[anti-Late serum]]
 
*[[anti-Late serum]]
 
*[[anti-NANP hybridoma supernatant]]
 
*[[anti-NANP hybridoma supernatant]]
 +
 +
===Plasmids===
 +
===Viruses===
 +
===Cells===
  
 
== Links ==
 
== Links ==

Revision as of 10:04, 6 September 2006

JHSPHbanner.jpg


Current Projects

Adenovirus Vaccines

Ubiquination


Protocols

  • Adenovirus CaCl transfection
  • Generating hexon mutants in E. coli
  • Generating fiber mutants in E. coli
  • Generating late gene expression mutants in 293 cells
  • Purifying adenovius (banded stocks)
  • Titration of viral stocks (calculate pfu)
  • Calculate MOI
  • Protein preps of infected 293 cells for westerns
  • Western Blot
  • P32 label DNA

Materials

Antibodies

Plasmids

Viruses

Cells

Links

Gary Ketner

JHSPH Magazine