Difference between revisions of "Ketner Lab"

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== Current Projects ==
== Current Projects ==
'''Adenovirus Vaccines'''
'''Adenovirus Vaccines'''

Revision as of 07:06, 30 August 2006


Current Projects

Adenovirus Vaccines



  • Adenovirus CaCl transfection
  • Generating hexon mutants in E. coli
  • Generating fiber mutants in E. coli
  • Generating late gene expression mutants in 293 cells
  • Purifying adenovius (banded stocks)
  • Titration of viral stocks (calculate pfu)
  • Calculate MOI
  • Protein preps of infected 293 cells for westerns
  • Western Blots
  • P32 label DNA


Gary Ketner

JHSPH Magazine